Antibodies A Laboratory Manual, Second edition Book PDF, ePub eBook

Join our eBook service for unlimited books & audiobooks. Get a free 7 day subscription! ↓↓↓↓↓


Book Descriptions:
It has been 25 years since the now-classic Antibodies by Ed Harlow and David Lane appeared. While some things remain the same, many have changed. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:

· antibody purification and storage
· engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
· extensive labeling techniques
· new immunoblotting protocols
· the latest screening and labeling techniques

As Dr. Greenfield notes in his preface to this second edition:
"The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book...the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project."

I have the first edition. Why should I buy the second edition?
While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition.

To whom is this edition addressed? Is this a book for immunologists?
As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments-both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem.

Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.

Antibodies A Laboratory Manual, Second edition

From reader reviews:

Harry Fulford:

Do you have favorite book? Should you have, what is your favorite's book? Publication is very important thing for us to be aware of everything in the world. Each book has different aim as well as goal; it means that book has different type. Some people really feel enjoy to spend their time to read a book. They are really reading whatever they consider because their hobby is definitely reading a book. Consider the person who don't like reading a book? Sometime, individual feel need book whenever they found difficult problem or perhaps exercise. Well, probably you will want this Antibodies A Laboratory Manual, Second edition.

Jack Bemis:

Book is written, printed, or outlined for everything. You can recognize everything you want by a e-book. Book has a different type. As you may know that book is important matter to bring us around the world. Close to that you can your reading talent was fluently. A publication Antibodies A Laboratory Manual, Second edition will make you to be smarter. You can feel far more confidence if you can know about anything. But some of you think that open or reading the book make you bored. It isn't make you fun. Why they may be thought like that? Have you seeking best book or suited book with you?

Michael Emery:

Spent a free the perfect time to be fun activity to do! A lot of people spent their leisure time with their family, or their own friends. Usually they carrying out activity like watching television, gonna beach, or picnic inside park. They actually doing same every week. Do you feel it? Do you need to something different to fill your free time/ holiday? Might be reading a book is usually option to fill your cost-free time/ holiday. The first thing you will ask may be what kinds of reserve that you should read. If you want to consider look for book, may be the guide untitled Antibodies A Laboratory Manual, Second edition can be excellent book to read. May be it could be best activity to you.

Lila Costillo:

You are able to spend your free time to read this book this book. This Antibodies A Laboratory Manual, Second edition is simple bringing you can read it in the park, in the beach, train and soon. If you did not have got much space to bring the actual printed book, you can buy the particular e-book. It is make you better to read it. You can save the book in your smart phone. Therefore there are a lot of benefits that you will get when you buy this book.